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Information resources of health education , disease knowledge and drug knowledge extracted by seconda-ry development fromhospital electronic librarywere disseminated on the Website , WIFI and WeChat platforms of People's Armed Police for the self-learning of users according to the demand of health dissemination and education in hospitals and health education at grass-roots level, in order to improve the efficiency and service of health edu-cation, solve the lack of resources and talents for health education, and to enhance the health service support ability for People's Armed Police.
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Objective To evaluate the value of circular anastomotic stapler for sigmoid colostomy in laparoscopy abdominoperi-neal resection .Methods 62 patients of low colorectal cancer treated in our hospital from October 2010 to February 2013 was retro-spectively analyzed .All the patients were treated by laparoscopy abdominoperineal resection ,34 patients received sigmoid colostomy with circular anastomotic stapler(group A) ,and the other 28 patients received sigmoid colostomy with conventional suturing tech-niques(group B) .The medical records including operation time ,the time of return of bowl function ,postoperative hospital stay and postoperative complication rate were analysed statistically .Results All patients received the operations successfully .No conversion to open procedure and no operative death occured in two groups .The result of statistical analysis showed that in group A ,the opera-tion time ,the time of return of bowl function ,postoperative hospital stay time were shorter than group B ,and the rate of edema of sigmoid in group A were lower than group B(P<0 .05) .Conclusion The application of circular anastomotic stapler for sigmoid co-lostomy in LAPR is a safe ,effective and minimally invasive technique ,which can shorten operation time ,postoperative hospital stay and reduce the related complications of colostomy .
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Objective To make better use of the antitumor effects of TNF related apopotosis inducing ligand (TRAIL) ,we con-struct a new fusion protein including TRAIL ,for the sake of improving its purity and targeting to hepatocytes .Methods Fusion protein concluding His label protein and human recombinant TRAIL which linked by constructed specifically u-PA cleavage site . TRAIL protein was obtained by disintigrating from the fusion protein which had been purified .Results The purified His-rh-sTRAIL was demonstrated to be cleavable by u-PA and possess enhanced antitumor effect ,it′s toxicity to hepatocytes was reduced . Conclusion The fusion protein His-rhsTRAIL has antitumor effect on colon cancer cell with high efficiency ,highly targeting and low-toxicity .
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It has been known that the Alzheimer's disease(AD)is related closely with a synaptic failure,and the p21-activated kinase(PAK)is well documented to play an important role in the regulation of the synaptie functions.However,the relationship between thePAK and the pathology of AD is unclear.In the present study,we examined the expressions of the PAK3(one subtype ofPAK),phospho-rylated-PAK(pPAK) and β-amyloid42(Aβ42,β-amyloid with 42 peptides)in an APP/PS1 double transgenie mouse model of AD andthe morphologies of geurOtlS in the hippocampus at different ages.The Western Blot results showed that the expression of PAK remainedunchanged,while,the expression of pPAK decreased largely at the age of 32 weeks and further decreased significantly with aging in thehippocampus of the APP/PS1 transgenic mouse.A1342 levels in the hippocampus were detected to increase as early as the age of 22 weeks,and kept the increase to continue with aging.The morphological results showed no obvious neuron loss in the sections of Nissl staining,while serious distonion and disorder of the dendrites of the hippocampal neurons were observed on the sections of Gelgi staining in theAPP/PS1 transgenic mouse.The present results suggested that it seemed something wrong in the processes of phospholization of PAK,butnot in the expression of the PAK itself;the toxic Aβ42 might affect the PAK in its phospholization,which in turn directly influence thedendritic development in the hippocampal neurons and cause the dendrites distorting and disordering.
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Objective To investigate the effect of photochemical gene transfection on colon cancer cells mediated by polycation.Methods Based on SW480 and HT29 colon carcinoma cell culture,the intracellular localization of meso-tetraphenylporphine disulfonate(TPPS_(2a)) were observed by laser confocal scanning microscopy(LCSM).Photochemically induced cytotoxicity of TPPS_(2a) on SW480 and HT29 cell lines was evaluated by MTT assay.By flow cytometry,the light-induced transfection of EGFP plasmids mediated by PEI was studied.Results TPPS_(2a) exhibited typical vesicular localization in two colon carcinoma cell lines.The photochemical cytotoxicity based on TPPS_(2a) increased with light dose.The light dose of IC_(50) was 22 mW/cm~(2) for 6 min for SW480 cells and for 12 min for HT29 cells(wavelength: 405 nm).The light treatment based on TPPS_(2a) induced an increase in transfection efficiency to(29.61?1.15) for SW480 cells and(23.47?0.62) for HT29 cells(P
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Objective To study the synergistic effects of Mitomycin C in photodynamic therapy for colonic carcinoma. Methods The colon carcinoma cell line SW800 was assigned to be treated with 002 ?g/ml Mitomycin C, or 2 ?g/ml PSD-007 (photosensitizer), or Mitomycin C and PSD-007 (PSD+MC group).The cells without any special treatment served as control. Fluorescence spectrophotometer and MTT assay were used to detect the content of intracellular photosensitizer and survival rate of SW700 cells under photodynamic therapy. The obtained data was analyzed with SPLM statistical software. Results After PSD was added for 4, 8, 16 and 24 h, intracellular PSD level of PSD+MC group increased significantly as compared with that of PSD group. The intracellular PSD contents of PSD group, PSD+MC group had a strong and negative correlation with cell survival rate respectively after photodynamic therapy, but the cell survival rate curve shifted to the left obviously. The best killing effects on SW700 cells of photodynamic therapy could be obtained when the SW700 cells had been exposed to photosensitizer for 8-16 h. Conclusion There is insignificant influence of lowconcentration of Mitomycin C alone on cell survival rate. The better synergistic effect of Mitomycin C on killing the colon carcimoma cells can be achieved by enhancing the intracellular content of photosensitizer for 6-8 h before photodynamic therapy.
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Objective To investigate ideal procedure of reconstruction for the digestive tract after total gastrectomy.Methods:After total gastrectomy,the 25 centimeters length jejunum with vessel pedicle was dissociated and was inosculated between esophagus distal and duodenum proximal end.This procedure is simple and meet the physiological need.Results:28 cases of patients were curved recently without complications and death.All patients wre followed-up long term survey that they had no intestinal symptoms,reverse esophagitis and nutritional deficiency.Their body weights were increased significantly and could take part in labor.Conclusion:This procedure is the ideal reconstruction operation for the digestive tract after total gastrectomy.
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Eighteen patients with hemangioma of alimentary tract were treated in our hospital from 1980 to 1994 There were 11 males and 7 females,with average age of 48?18 years (ranged from 19 to 76 years) Of them,14 patients were treated with operation,2 with hemangioma of rectum were treated with ligation with snare.All of the 16 patients were cured. The etiology,pathology,diagnosis and management are discussed in this paper The experiences of finding small hemangiomas of jejunum and ileum during operation are also introduced in the paper